Substrate concentration coursework

For example, if there were more molecules of yeast, the rate of reaction would increase because there would be more collisions between enzyme and substrate Substrate concentration coursework.

It could be repeated more times to help get rid of any anomalies. If two enzymes, in different pathways, compete for the same substrate, then knowing the values of Km and Vmax for both enzymes permits prediction of the metabolic fate of the substrate and the relative amount that will flow through each pathway under various conditions.

The line of best fit also suggests values of concentrations which I have not investigated. Bio-inspired Robotics Virtual Labs Remote Trigger This remote-triggerable online laboratory will teach experiments and offer to introduce biorobotics and neuronal robot techniques.

So in cm3, the actual volume could have been either However, in reality it is impossible to keep all the variables precisely the same. This could have affected my results for Substrate concentration coursework reasons.

It was very hard to insert the small 5cm3 beaker into the conical flask, and when it came to tipping it over, some of the substrate was still trapped inside the beaker.

However, some oxygen was displaced in the gas syringe and I had to solve this by subtracting this small amount from the volumes produced in each of the reactions.

Effect of Substrate Concentration on the Rate of Activity of Catalase

This means the amount of substrate could have been different for each repeat, even though I used the same concentration. As I have mentioned earlier, this decreases in steps of 3. The volume of gas in the test tube to start with is slightly affected by the amount which the bung is pushed down each time, if the bung is pushed down further then the volume in the tube will be less so the 30cm3 of gas is reached faster.

For practical purposes, Km is the concentration of substrate which permits the enzyme to achieve half Vmax. The addition or removal of one species affects many other species with which it might compete for,or provide food. Surface Area of Yeast Molecules I ground up the yeast to try to make the surface area as similar as possible because surface area is a major factor in my experiment.

Though in theory, this should be the trend, my results did not demonstrate this pattern. I did this by only using pipettes, which have a very small apparatus error when compared to beakers.

Effect of Substrate Concentration on the Rate of Activity of Catalase

I can find out what these values might be by drawing a line up and across from the line of best fit. Consistent Temperature Temperature is a major factor which affects the rate of reaction. This is because at higher temperatures, molecules of both enzyme and substrate have more kinetic energy and collide more often.

The reaction is exothermic, meaning heat is produced in the reaction. For example, the final average volumes of gas were as follows: Apparatus Limitations Firstly, there were limitations on the apparatus that I used.

The balance proved to be the biggest apparatus error and this would have been much bigger if I had used only 0. Overall, there is a pattern showing a constant trend in that as the concentration decreases the rate of reaction decreases too, and that the overall volume of gas evolved also decreases.

Each piece of apparatus has an apparatus error with an upper and lower limit. Cell biology Virtual Lab I Cell biology is an exciting and dynamic area that helps discover the fascinating world of cells. Ecosystems have an extremely complex web of cause and effect. The focus is on practical skills in using simple electronics to reinforce application of bio-inspired ideas.

I did this by only using pipettes, which have a very small apparatus error when compared to beakers. This is probably due to an experimental error involving one of the factors mentioned above.

I will use exactly the same method as I did previously, and since I still have some yeast left, I can still use the same batch of yeast. If an enzyme is to be used to determine the concentration of substrate in a sample e.

Bioinformatics Virtual Lab II This virtual laboratory is for undergraduate and postgraduate students to get a deeper understanding on the analysis of sequence data, its alignment and the evolutionary relationship.Substrate concentration is the amount of substrate present that can be turned into product and is most commonly measured in molarity (moles per liter).

The concentration of substrates is often. The conditions selected to measure the activity of an enzyme would not be the same as those selected to measure the concentration of its substrate.

Several factors affect the rate at which enzymatic reactions proceed – temperature, pH, enzyme concentration, substrate concentration, and the presence of any inhibitors or activators. Substrate Concentration. So how many scrambled eggs can you make?

Well, that depends on the number of eggs you have. The amount of eggs is your concentration. Substrate concentration is the amount of substrate present that can be turned into product. The. A) At saturating levels of substrate, the rate of an enzyme-catalyzed reaction is proportional to the enzyme concentration.

B) If enough substrate is added, the normal Vmax of a reaction can be attained even in the presence of a competitive inhibitor. View the step-by-step solution to: 4. At an unknown substrate concentration an enzyme exhibits a rate of umol mint.

The KM for thi enzyme - substrate reaction is 7 23 M. Knowledge application - use your knowledge of substrate concentration to answer questions about how substrate binding occurs and what substrate concentration determines Additional Learning Substrate Concentration: Definition and Effect on Enzyme Activity is the lesson you need to refer to if you're interested in learning more about this topic.

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Substrate concentration coursework
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